› 3nbz

X-ray diffraction
2.8Å resolution

Crystal structure of the HIV-1 Rev NES-CRM1-RanGTP nuclear export complex (crystal I)

Released:
DOI: 10.2210/pdb3nbz/pdb
PDB entry 3nbz coloured by chain, front view.
PDB entry 3nbz coloured by chain, side view.
PDB entry 3nbz coloured by chain, top view.
Source organisms:
Primary publication:
NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1.
Güttler T, Madl T, Neumann P, Deichsel D, Corsini L, Monecke T, Ficner R, Sattler M, Görlich D
Nat Struct Mol Biol 17 1367-76 (2010)
PMID: 20972448

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-131824 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Exportin-1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 1073 amino acids
Theoretical weight: 123.37 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6P5F9 (Residues: 1-1071; Coverage: 100%)
Gene names: Crm1, Xpo1
Sequence domains:
Snurportin-1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 362 amino acids
Theoretical weight: 41.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95149 (Residues: 15-360; Coverage: 96%)
Gene names: RNUT1, SNUPN, SPN1
Sequence domains: Snurportin1
Structure domains: DNA ligase/mRNA capping enzyme
GTP-binding nuclear protein Ran Chains: C, F
Molecule details ›
Chains: C, F
Length: 176 amino acids
Theoretical weight: 20.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62826 (Residues: 5-180; Coverage: 82%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 72.66Å b: 224.617Å c: 164.021Å
α: 90° β: 100.82° γ: 90°
R-values:
R R work R free
0.229 0.226 0.285
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Components and regulation of nuclear transport processes.
Cautain et al. (2015)
25 more

11 mentions without citation

Structural Basis of Targeting the Exportin CRM1 in Cancer.
Dickmanns et al. (2015)
10 more