› 3ndf

X-ray diffraction
2.7Å resolution

Cleaved antitrypsin with P8-P6 Asp

Released:
DOI: 10.2210/pdb3ndf/pdb
PDB entry 3ndf coloured by chain, front view.
PDB entry 3ndf coloured by chain, side view.
PDB entry 3ndf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Loop-sheet mechanism of serpin polymerization tested by reactive center loop mutations.
Yamasaki M, Sendall TJ, Harris LE, Lewis GM, Huntington JA
J Biol Chem 285 30752-8 (2010)
PMID: 20667823

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133785 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-1-antitrypsin Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 38.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01009 (Residues: 46-382; Coverage: 86%)
Gene names: AAT, PI, PRO0684, PRO2209, SERPINA1
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:
Short peptide from AAT Chain: B
Molecule details ›
Chain: B
Length: 36 amino acids
Theoretical weight: 4.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01009 (Residues: 383-418; Coverage: 9%)
Gene names: AAT, PI, PRO0684, PRO2209, SERPINA1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 37.45Å b: 83.27Å c: 61.09Å
α: 90° β: 99.66° γ: 90°
R-values:
R R work R free
0.217 0.211 0.268
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Serpin structure, function and dysfunction.
Huntington JA. (2011)
15 other articles

3 mentions without citation

High-throughput sequencing for the detection of the bacterial and fungal diversity in Mongolian naturally fermented cow's milk in Russia.
Liu et al. (2015)
2 more