› 3nh7

X-ray diffraction
2.7Å resolution

Crystal structure of the neutralizing Fab fragment AbD1556 bound to the BMP type I receptor IA

Released:
DOI: 10.2210/pdb3nh7/pdb
PDB entry 3nh7 coloured by chain, front view.
PDB entry 3nh7 coloured by chain, side view.
PDB entry 3nh7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A selection fit mechanism in BMP receptor IA as a possible source for BMP ligand-receptor promiscuity.
Harth S, Kotzsch A, Hu J, Sebald W, Mueller TD
PLoS One 5 (2010)
PMID: 20927405

Function and Biology Details

Reaction catalysed: 
ATP + [receptor-protein] = ADP + [receptor-protein] phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234023 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody fragment Fab AbD1556, heavy chain Chains: H, I, J, K
Molecule details ›
Chains: H, I, J, K
Length: 234 amino acids
Theoretical weight: 25.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Antibody fragment Fab AbD1556, light chain Chains: L, M, N, O
Molecule details ›
Chains: L, M, N, O
Length: 213 amino acids
Theoretical weight: 22.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Bone morphogenetic protein receptor type-1A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 129 amino acids
Theoretical weight: 14.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36894 (Residues: 24-152; Coverage: 25%)
Gene names: ACVRLK3, ALK3, BMPR1A
Sequence domains: Activin types I and II receptor domain
Structure domains: CD59

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 89.32Å b: 129.255Å c: 100.239Å
α: 90° β: 92.27° γ: 90°
R-values:
R R work R free
0.236 0.234 0.28
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structural Biology and Evolution of the TGF-β Family.
Hinck et al. (2016)
1 more

9 mentions without citation

H7N9 influenza virus neutralizing antibodies that possess few somatic mutations.
Thornburg et al. (2016)
8 more