› 3nmd

X-ray diffraction
2.27Å resolution

Crystal structure of the leucine zipper domain of cGMP dependent protein kinase I beta

Released:
DOI: 10.2210/pdb3nmd/pdb
PDB entry 3nmd coloured by chain, front view.
PDB entry 3nmd coloured by chain, side view.
PDB entry 3nmd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A crystal structure of the cyclic GMP-dependent protein kinase I{beta} dimerization/docking domain reveals molecular details of isoform-specific anchoring.
Casteel DE, Smith-Nguyen EV, Sankaran B, Roh SH, Pilz RB, Kim C
J Biol Chem 285 32684-32688 (2010)
PMID: 20826808

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent protein kinase 1 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 72 amino acids
Theoretical weight: 8.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q13976 (Residues: 4-47; Coverage: 6%)
Gene names: PRKG1, PRKG1B, PRKGR1A, PRKGR1B
Sequence domains: Coiled-coil N-terminus of cGMP-dependent protein kinase
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

2 bound ligands:
Ligand HEZ 7 x HEZ
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2221
Unit cell:
a: 62.774Å b: 77.312Å c: 148.616Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.248
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Cyclic nucleotide phosphodiesterase (PDE) isozymes as targets of the intracellular signalling network: benefits of PDE inhibitors in various diseases and perspectives for future therapeutic developments.
Keravis et al. (2012)
9 more

9 mentions without citation

Targeted proteolysis of plectin isoform 1a accounts for hemidesmosome dysfunction in mice mimicking the dominant skin blistering disease EBS-Ogna.
Walko et al. (2011)
8 more