› 3nsz

X-ray diffraction
1.3Å resolution

Human CK2 catalytic domain in complex with AMPPN

Released:
DOI: 10.2210/pdb3nsz/pdb
PDB entry 3nsz coloured by chain, front view.
PDB entry 3nsz coloured by chain, side view.
PDB entry 3nsz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of CX-4945 binding to human protein kinase CK2.
Ferguson AD, Sheth PR, Basso AD, Paliwal S, Gray K, Fischmann TO, Le HV
FEBS Lett 585 104-10 (2011)
PMID: 21093442

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase II subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 39.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P68400 (Residues: 2-331; Coverage: 84%)
Gene names: CK2A1, CSNK2A1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 2 x SO4
Ligand MG 2 x MG
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 49.382Å b: 62.026Å c: 117.061Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Protein kinase CK2 inhibitors: a patent review.
Cozza et al. (2012)
7 more

12 mentions without citation

Coumestrol from the national cancer Institute's natural product library is a novel inhibitor of protein kinase CK2.
Liu et al. (2013)
11 more