› 3nx0

X-ray diffraction
2.04Å resolution

Hinge-loop mutation can be used to control 3D domain swapping and amyloidogenesis of human cystatin C

Released:
DOI: 10.2210/pdb3nx0/pdb
PDB entry 3nx0 coloured by chain, front view.
PDB entry 3nx0 coloured by chain, side view.
PDB entry 3nx0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Hinge-loop mutation can be used to control 3D domain swapping and amyloidogenesis of human cystatin C.
Orlikowska M, Jankowska E, Kołodziejczyk R, Jaskólski M, Szymańska A
J Struct Biol 173 406-13 (2011)
PMID: 21074623

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystatin-C Chains: A, B
Molecule details ›
Chains: A, B
Length: 120 amino acids
Theoretical weight: 13.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01034 (Residues: 27-146; Coverage: 100%)
Gene name: CST3
Sequence domains: Cystatin domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P61
Unit cell:
a: 76.316Å b: 76.316Å c: 97.723Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.225
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Biological Activities of Secretory RNases: Focus on Their Oligomerization to Design Antitumor Drugs.
Gotte et al. (2019)
5 more

6 mentions without citation

Influence of point mutations on the stability, dimerization, and oligomerization of human cystatin C and its L68Q variant.
Szymańska et al. (2012)
5 more