› 3o0x

X-ray diffraction
2.01Å resolution

Structural basis of carbohydrate recognition by calreticulin

Released:
DOI: 10.2210/pdb3o0x/pdb
PDB entry 3o0x coloured by chain, front view.
PDB entry 3o0x coloured by chain, side view.
PDB entry 3o0x coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of carbohydrate recognition by calreticulin.
Kozlov G, Pocanschi CL, Rosenauer A, Bastos-Aristizabal S, Gorelik A, Williams DB, Gehring K
J Biol Chem 285 38612-20 (2010)
PMID: 20880849

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Calreticulin Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 31.3 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14211 (Residues: 18-206, 301-368; Coverage: 64%)
Gene name: Calr
Sequence domains: Calreticulin family
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, GLC
Carbohydrate polymer
1 bound ligand:
Ligand CA 2 x CA
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 74.619Å b: 43.225Å c: 84.632Å
α: 90° β: 96.05° γ: 90°
R-values:
R R work R free
0.2 0.191 0.24
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Protein folding and quality control in the endoplasmic reticulum: Recent lessons from yeast and mammalian cell systems.
Brodsky et al. (2011)
19 more

8 mentions without citation

Calnexin cycle - structural features of the ER chaperone system.
Kozlov et al. (2020)
7 more