› 3o2d

X-ray diffraction
2.19Å resolution

Crystal structure of HIV-1 primary receptor CD4 in complex with a potent antiviral antibody

Released:
DOI: 10.2210/pdb3o2d/pdb
PDB entry 3o2d coloured by chain, front view.
PDB entry 3o2d coloured by chain, side view.
PDB entry 3o2d coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of HIV-1 primary receptor CD4 in complex with a potent antiviral antibody.
Freeman MM, Seaman MS, Rits-Volloch S, Hong X, Kao CY, Ho DD, Chen B
Structure 18 1632-41 (2010)
PMID: 21134642

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208507 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ibalizumab light chain Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.25 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
ibalizumab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 24.3 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
T-cell surface glycoprotein CD4 Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 21.03 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01730 (Residues: 26-207; Coverage: 42%)
Gene name: CD4
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 52.164Å b: 66.266Å c: 266.659Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.224
Expression systems:
  • Mus musculus
  • Spodoptera frugiperda

Quick links

Citations

15 review citations

Molecular Mechanism of HIV-1 Entry.
Chen B. (2019)
14 more

14 mentions without citation

Antibody structure determination using a combination of homology modeling, energy-based refinement, and loop prediction.
Zhu et al. (2014)
13 more