› 3o2g

X-ray diffraction
1.78Å resolution

Crystal Structure of Human gamma-butyrobetaine,2-oxoglutarate dioxygenase 1 (BBOX1)

Released:
DOI: 10.2210/pdb3o2g/pdb
PDB entry 3o2g coloured by chain, front view.
PDB entry 3o2g coloured by chain, side view.
PDB entry 3o2g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and mechanistic studies on γ-butyrobetaine hydroxylase.
Leung IK, Krojer TJ, Kochan GT, Henry L, von Delft F, Claridge TD, Oppermann U, McDonough MA, Schofield CJ
Chem Biol 17 1316-24 (2010)
PMID: 21168767

Function and Biology Details

Reaction catalysed: 
4-trimethylammoniobutanoate + 2-oxoglutarate + O(2) = 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-butyrobetaine dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 44.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O75936 (Residues: 1-387; Coverage: 100%)
Gene names: BBH, BBOX, BBOX1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand OGA 1 x OGA
Ligand NM2 1 x NM2
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: R32
Unit cell:
a: 107.386Å b: 107.386Å c: 204.919Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.147 0.176
Expression system: Trichoplusia ni

Quick links

Citations

8 review citations

Inhibition of 2-oxoglutarate dependent oxygenases.
Rose et al. (2011)
7 more

12 mentions without citation

Imposing function down a (cupin)-barrel: secondary structure and metal stereochemistry in the αKG-dependent oxygenases.
Hangasky et al. (2013)
11 more