› 3o47

X-ray diffraction
2.8Å resolution

Crystal structure of ARFGAP1-ARF1 fusion protein

Released:
DOI: 10.2210/pdb3o47/pdb
PDB entry 3o47 coloured by chain, front view.
PDB entry 3o47 coloured by chain, side view.
PDB entry 3o47 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Wang H, Tong Y, Nedyalkova L, Tempel W, Guan X, Crombet L, Arrowsmith CH, Edwards AM, Bountra C, Weigelt J, Bochkarev A, Park H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylation factor 1; ADP-ribosylation factor GTPase-activating protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 37.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8N6T3 (Residues: 1-140; Coverage: 35%)
  • Canonical: P84077 (Residues: 11-181; Coverage: 95%)
Gene names: ARF1, ARF1GAP, ARFGAP1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: I212121
Unit cell:
a: 79.298Å b: 129.161Å c: 157.685Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.243
Expression system: Escherichia coli BL21

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