› 3o77

X-ray diffraction
2.35Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143904 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1; Green fluorescent protein; Myosin light chain kinase, smooth muscle, deglutamylated form Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 46.48 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P11799 (Residues: 1730-1763; Coverage: 2%)
  • Canonical: P42212 (Residues: 2-170, 191-192; Coverage: 70%)
  • Canonical: P0DP23 (Residues: 23-42, 45-149; Coverage: 84%)
Gene names: CALM, CALM1, CAM, CAM1, GFP, Mylk
Sequence domains:
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand SO4 2 x SO4
Ligand CL 1 x CL
1 modified residue:
Ligand CR2 1 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21212
Unit cell:
a: 92.499Å b: 106.941Å c: 43.265Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.198 0.274
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Optogenetic experimentation on astrocytes.
Figueiredo et al. (2011)
3 more

3 mentions without citation

Mechanical and chemical activation of GPR68 probed with a genetically encoded fluorescent reporter.
Ozkan et al. (2021)
2 more