› 3o78

X-ray diffraction
2.6Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-143904 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1; Green fluorescent protein; Myosin light chain kinase, smooth muscle, deglutamylated form Chains: A, B
Molecule details ›
Chains: A, B
Length: 415 amino acids
Theoretical weight: 46.49 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P11799 (Residues: 1730-1763; Coverage: 2%)
  • Canonical: P42212 (Residues: 2-170, 191-192; Coverage: 70%)
  • Canonical: P0DP23 (Residues: 23-42, 45-149; Coverage: 84%)
Gene names: CALM, CALM1, CAM, CAM1, GFP, Mylk
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
1 modified residue:
Ligand CR2 2 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 46.374Å b: 101.635Å c: 82.211Å
α: 90° β: 91.45° γ: 90°
R-values:
R R work R free
0.245 0.242 0.316
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Genetically encoded Ca(2+) indicators: properties and evaluation.
Pérez Koldenkova et al. (2013)
3 more

2 mentions without citation

The 'mitoflash' probe cpYFP does not respond to superoxide.
Schwarzländer et al. (2014)
1 more