› 3oa6

X-ray diffraction
2.35Å resolution

Human MSL3 Chromodomain bound to DNA and H4K20me1 peptide

Released:
DOI: 10.2210/pdb3oa6/pdb
PDB entry 3oa6 coloured by chain, front view.
PDB entry 3oa6 coloured by chain, side view.
PDB entry 3oa6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Corecognition of DNA and a methylated histone tail by the MSL3 chromodomain.
Kim D, Blus BJ, Chandra V, Huang P, Rastinejad F, Khorasanizadeh S
Nat Struct Mol Biol 17 1027-9 (2010)
PMID: 20657587

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116640 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
MSL complex subunit 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 13.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N5Y2 (Residues: 1-101; Coverage: 19%)
Gene names: MSL3, MSL3L1
Sequence domains: RNA binding activity-knot of a chromodomain
Structure domains: SH3 type barrels.
H4 peptide monomethylated at lysine 20 Chain: G
Molecule details ›
Chain: G
Length: 24 amino acids
Theoretical weight: 2.69 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*CP*TP*AP*GP*GP*TP*CP*AP*AP*AP*GP*GP*TP*CP*A)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 16 nucleotides
Theoretical weight: 4.95 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*TP*GP*AP*CP*CP*TP*TP*TP*GP*AP*CP*CP*TP*AP*G)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 16 nucleotides
Theoretical weight: 4.85 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand IMD 1 x IMD
1 modified residue:
Ligand MLZ 1 x MLZ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 37.976Å b: 44.094Å c: 67.5Å
α: 81.35° β: 89.84° γ: 90.04°
R-values:
R R work R free
0.229 0.226 0.277
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

24 review citations

Perceiving the epigenetic landscape through histone readers.
Musselman et al. (2012)
23 more

7 mentions without citation

Readout of epigenetic modifications.
Patel et al. (2013)
6 more