› 3od4

X-ray diffraction
2.2Å resolution

Crystal Structure of Factor Inhibiting HIF-1 Alpha Complexed with Inhibitor

Released:
DOI: 10.2210/pdb3od4/pdb
PDB entry 3od4 coloured by chain, front view.
PDB entry 3od4 coloured by chain, side view.
PDB entry 3od4 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: King ONF, Bashford-Rogers R, Maloney DJ, Jadhav A, Heightman TD, Simeonov A, Clifton IJ, McDonough MA, Schofield CJ

Function and Biology Details

Reactions catalysed: 
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
[Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxia-inducible factor 1-alpha inhibitor Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 40.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NWT6 (Residues: 1-349; Coverage: 100%)
Gene names: FIH1, HIF1AN
Sequence domains: Cupin-like domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand 8XQ 1 x 8XQ
Ligand SO4 3 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P41212
Unit cell:
a: 86.4Å b: 86.4Å c: 147.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.212
Expression system: Escherichia coli

Quick links