› 3od5

X-ray diffraction
1.6Å resolution

Crystal structure of active caspase-6 bound with Ac-VEID-CHO

Released:
DOI: 10.2210/pdb3od5/pdb
PDB entry 3od5 coloured by chain, front view.
PDB entry 3od5 coloured by chain, side view.
PDB entry 3od5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human caspase 6 reveal a new mechanism for intramolecular cleavage self-activation.
Wang XJ, Cao Q, Liu X, Wang KT, Mi W, Zhang Y, Li LF, LeBlanc AC, Su XD
EMBO Rep 11 841-7 (2010)
PMID: 20890311

Function and Biology Details

Reaction catalysed: 
Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157269 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Caspase-6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 32.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55212 (Residues: 24-293; Coverage: 92%)
Gene names: CASP6, MCH2
Sequence domains: Caspase domain
Structure domains: Rossmann fold
peptide aldehyde inhibitor AC-VEID-CHO Chains: C, D
Molecule details ›
Chains: C, D
Length: 5 amino acids
Theoretical weight: 485 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CAC 8 x CAC
1 modified residue:
Ligand ASA 2 x ASA

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 55.464Å b: 89.589Å c: 61.145Å
α: 90° β: 111.67° γ: 90°
R-values:
R R work R free
0.156 0.155 0.189
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Huntington's disease: from molecular pathogenesis to clinical treatment.
Ross et al. (2011)
8 more

20 mentions without citation

Small Molecule Active Site Directed Tools for Studying Human Caspases.
Poreba et al. (2015)
19 more