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X-ray diffraction
2.9Å resolution

Crystal Structure of the DH/PH Domains of p115-RhoGEF

Released:
DOI: 10.2210/pdb3odo/pdb
PDB entry 3odo coloured by chain, front view.
PDB entry 3odo coloured by chain, side view.
PDB entry 3odo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Modulation of a GEF switch: Autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoREF
Chen Z, Guo L, Sprang SR, Sternweis PC
Protein Sci. 20 107-117 (2011)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187799 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho guanine nucleotide exchange factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 43.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92888 (Residues: 395-766; Coverage: 41%)
Gene name: ARHGEF1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41
Unit cell:
a: 106.049Å b: 106.049Å c: 125.973Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.272
Expression system: Escherichia coli BL21(DE3)

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