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X-ray diffraction
3.2Å resolution

Crystal Structure of an N-terminally Truncated Linker-DH/PH Domains of p115-RhoGEF

Released:
DOI: 10.2210/pdb3odx/pdb
PDB entry 3odx coloured by chain, front view.
PDB entry 3odx coloured by chain, side view.
PDB entry 3odx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Modulation of a GEF switch: Autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoREF
Chen Z, Guo L, Sprang SR, Sternweis PC
Protein Sci. 20 107-117 (2011)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187799 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho guanine nucleotide exchange factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 417 amino acids
Theoretical weight: 48.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92888 (Residues: 353-766; Coverage: 45%)
Gene name: ARHGEF1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P32
Unit cell:
a: 111.501Å b: 111.501Å c: 97.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.225 0.274
Expression system: Escherichia coli BL21(DE3)

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