3ohx

X-ray diffraction
3.5Å resolution

Molecular Basis for Complement Recognition and Inhibition Determined by Crystallographic Studies of the Staphylococcal Complement Inhibitor (SCIN) Bound to C3c and C3b

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-133825 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Complement C3 beta chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 645 amino acids
Theoretical weight: 71.39 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 23-667; Coverage: 39%)
Gene names: C3, CPAMD1
Sequence domains:
Structure domains:
Complement C3c alpha' chain fragment 1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 206 amino acids
Theoretical weight: 23.62 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 749-954; Coverage: 13%)
Gene names: C3, CPAMD1
Sequence domains:
Structure domains:
Complement C3c alpha' chain fragment 2 Chains: C, F
Molecule details ›
Chains: C, F
Length: 343 amino acids
Theoretical weight: 39.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 1321-1663; Coverage: 21%)
Gene names: C3, CPAMD1
Sequence domains:
Structure domains:
Staphylococcal complement inhibitor Chains: M, P
Molecule details ›
Chains: M, P
Length: 88 amino acids
Theoretical weight: 10.06 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: Q931M7 (Residues: 32-116; Coverage: 100%)
Gene names: SAV1942, scn
Sequence domains: Staphylococcal complement inhibitor SCIN
Structure domains: Substrate Binding Domain Of Dnak; Chain:A; Domain 2

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21212
Unit cell:
a: 231.159Å b: 231.497Å c: 68.885Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.275 0.274 0.294
Expression system: Escherichia coli