› 3ojv

X-ray diffraction
2.6Å resolution

Crystal Structure of FGF1 complexed with the ectodomain of FGFR1c exhibiting an ordered ligand specificity-determining betaC'-betaE loop

Released:
DOI: 10.2210/pdb3ojv/pdb
PDB entry 3ojv coloured by chain, front view.
PDB entry 3ojv coloured by chain, side view.
PDB entry 3ojv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Plasticity in interactions of fibroblast growth factor 1 (FGF1) N terminus with FGF receptors underlies promiscuity of FGF1.
Beenken A, Eliseenkova AV, Ibrahimi OA, Olsen SK, Mohammadi M
J Biol Chem 287 3067-78 (2012)
PMID: 22057274

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138604 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Fibroblast growth factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 15.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05230 (Residues: 21-155; Coverage: 87%)
Gene names: FGF1, FGFA
Sequence domains: Fibroblast growth factor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)
Fibroblast growth factor receptor 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 226 amino acids
Theoretical weight: 25.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11362 (Residues: 142-365; Coverage: 28%)
Gene names: BFGFR, CEK, FGFBR, FGFR1, FLG, FLT2, HBGFR
Sequence domains: Immunoglobulin I-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: SGN, IDS, UAP
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P1
Unit cell:
a: 53.324Å b: 53.421Å c: 80.463Å
α: 106.44° β: 106.4° γ: 94.44°
R-values:
R R work R free
0.251 0.251 0.309
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The Fibroblast Growth Factor signaling pathway.
Ornitz et al. (2015)
5 more

13 mentions without citation

FGFR1 mutations cause Hartsfield syndrome, the unique association of holoprosencephaly and ectrodactyly.
Simonis et al. (2013)
12 more