› 3olc

X-ray diffraction
2.4Å resolution

Crystal structure of the N-terminal region of TopBP1

Released:
DOI: 10.2210/pdb3olc/pdb
PDB entry 3olc coloured by chain, front view.
PDB entry 3olc coloured by chain, side view.
PDB entry 3olc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the N-terminal region of human Topoisomerase IIβ binding protein 1.
Huo YG, Bai L, Xu M, Jiang T
Biochem Biophys Res Commun 401 401-5 (2010)
PMID: 20858457

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 2-binding protein 1 Chain: X
Molecule details ›
Chain: X
Length: 298 amino acids
Theoretical weight: 35.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92547 (Residues: 1-290; Coverage: 19%)
Gene names: KIAA0259, TOPBP1
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P213
Unit cell:
a: 115.73Å b: 115.73Å c: 115.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.258
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Regulating DNA replication in eukarya.
Siddiqui et al. (2013)
4 more

3 mentions without citation

Discovery and Validation of Lmj_04_BRCT Domain, a Novel Therapeutic Target: Identification of Candidate Drugs for Leishmaniasis.
Peña-Guerrero et al. (2021)
2 more