› 3olh

X-ray diffraction
2.5Å resolution

Human 3-mercaptopyruvate sulfurtransferase

Released:
DOI: 10.2210/pdb3olh/pdb
PDB entry 3olh coloured by chain, front view.
PDB entry 3olh coloured by chain, side view.
PDB entry 3olh coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Karlberg T, Collins R, Arrowsmith CH, Berglund H, Bountra C, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson I, Kotenyova T, Kouznetsova E, Moche M, Nordlund P, Nyman T, Persson C, Schutz P, Sehic A, Siponen MI, Thorsell AG, Tresaugues L, Van Den Berg S, Wahlberg E, Weigelt J, Welin M, Schuler H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(1a) 2-oxo-3-sulfanylpropanoate + [3-mercaptopyruvate sulfurtransferase]-L-cysteine = pyruvate + [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-mercaptopyruvate sulfurtransferase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 33.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25325 (Residues: 11-289; Coverage: 94%)
Gene names: MPST, TST2
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: R32
Unit cell:
a: 171.314Å b: 171.314Å c: 75.764Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.221 0.27
Expression system: Escherichia coli BL21(DE3)

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