› 3onw

X-ray diffraction
2.38Å resolution

Structure of a G-alpha-i1 mutant with enhanced affinity for the RGS14 GoLoco motif.

Released:
DOI: 10.2210/pdb3onw/pdb
PDB entry 3onw coloured by chain, front view.
PDB entry 3onw coloured by chain, side view.
PDB entry 3onw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural determinants of affinity enhancement between GoLoco motifs and G-protein alpha subunit mutants.
Bosch DE, Kimple AJ, Sammond DW, Muller RE, Miley MJ, Machius M, Kuhlman B, Willard FS, Siderovski DP
J Biol Chem 286 3351-8 (2011)
PMID: 21115486

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128999 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63096 (Residues: 31-354; Coverage: 92%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Structure domains:
Regulator of G-protein signaling 14 Chains: C, D
Molecule details ›
Chains: C, D
Length: 36 amino acids
Theoretical weight: 4.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O43566 (Residues: 497-532; Coverage: 6%)
Gene name: RGS14
Sequence domains: GoLoco motif

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P2221
Unit cell:
a: 70.392Å b: 83.679Å c: 190.148Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.265
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Applications of isothermal titration calorimetry in pure and applied research--survey of the literature from 2010.
Ghai et al. (2012)
2 more

4 mentions without citation

Evaluating caveolin interactions: do proteins interact with the caveolin scaffolding domain through a widespread aromatic residue-rich motif?
Byrne et al. (2012)
3 more