› 3orh

X-ray diffraction
1.86Å resolution

Human guanidinoacetate N-methyltransferase with SAH

Released:
DOI: 10.2210/pdb3orh/pdb
PDB entry 3orh coloured by chain, front view.
PDB entry 3orh coloured by chain, side view.
PDB entry 3orh coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Dong A, Wu H, Zeng H, Loppnau P, Sundstrom M, Arrowsmith CH, Edwards AM, Bochkarev A, Plotnikov AN, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanidinoacetate = S-adenosyl-L-homocysteine + creatine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171994 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanidinoacetate N-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 236 amino acids
Theoretical weight: 26.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14353 (Residues: 1-236; Coverage: 100%)
Gene name: GAMT
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 4 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P64
Unit cell:
a: 200.327Å b: 200.327Å c: 52.06Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.209 0.236
Expression system: Escherichia coli

Quick links