› 3ovu

X-ray diffraction
2.83Å resolution

Crystal Structure of Human Alpha-Haemoglobin Complexed with AHSP and the First NEAT Domain of IsdH from Staphylococcus aureus

Released:
DOI: 10.2210/pdb3ovu/pdb
PDB entry 3ovu coloured by chain, front view.
PDB entry 3ovu coloured by chain, side view.
PDB entry 3ovu coloured by chain, top view.
Source organisms:
Entry authors: Jacques DA, Krishna Kumar K, Caradoc-Davies TT, Langley DB, Mackay JP, Guss JM, Gell DA

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-159683 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Alpha-hemoglobin-stabilizing protein Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZD4 (Residues: 2-102; Coverage: 99%)
Gene names: AHSP, EDRF, ERAF
Sequence domains: Alpha-haemoglobin stabilising protein
Structure domains: AHSP
Iron-regulated surface determinant protein H Chain: B
Molecule details ›
Chain: B
Length: 164 amino acids
Theoretical weight: 18.9 KDa
Source organism: Staphylococcus aureus subsp. aureus MSSA476
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6G8J7 (Residues: 86-229; Coverage: 17%)
Gene names: SAS1657, harA, isdH, sasI
Sequence domains: Iron Transport-associated domain
Structure domains: Immunoglobulin-like
Hemoglobin subunit alpha Chain: C
Molecule details ›
Chain: C
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 68.638Å b: 71.659Å c: 82.058Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.252 0.28
Expression system: Escherichia coli

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