› 3oy5

X-ray diffraction
2.31Å resolution

The crystal structure of uPA complex with peptide inhibitor MH027 at pH7.4

Released:
DOI: 10.2210/pdb3oy5/pdb
PDB entry 3oy5 coloured by chain, front view.
PDB entry 3oy5 coloured by chain, side view.
PDB entry 3oy5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The binding mechanism of a peptidic cyclic serine protease inhibitor.
Jiang L, Svane AS, Sørensen HP, Jensen JK, Hosseini M, Chen Z, Weydert C, Nielsen JT, Christensen A, Yuan C, Jensen KJ, Nielsen NC, Malmendal A, Huang M, Andreasen PA
J Mol Biol 412 235-50 (2011)
PMID: 21802428

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133259 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 253 amino acids
Theoretical weight: 28.44 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00749 (Residues: 179-431; Coverage: 62%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
MH027 Chain: P
Molecule details ›
Chain: P
Length: 23 amino acids
Theoretical weight: 2.25 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R3
Unit cell:
a: 121.018Å b: 121.018Å c: 43.219Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.231
Expression systems:
  • Komagataella pastoris
  • Not provided

Quick links

Citations

1 review citation

Size does matter! Label-free detection of small molecule-protein interaction.
Fechner et al. (2014)
5 other articles

1 mention without citation

Structural Principles in the Development of Cyclic Peptidic Enzyme Inhibitors.
Xu et al. (2017)