› 3ozd

X-ray diffraction
2.1Å resolution

Crystal Structure of human 5'-deoxy-5'-methyladenosine phosphorylase in complex with pCl-phenylthioDADMeImmA

Released:
DOI: 10.2210/pdb3ozd/pdb
PDB entry 3ozd coloured by chain, front view.
PDB entry 3ozd coloured by chain, side view.
PDB entry 3ozd coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Ho M, Guan R, Almo SC, Schramm VL

Function and Biology Details

Reaction catalysed: 
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171545 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-methyl-5'-thioadenosine phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 31.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13126 (Residues: 1-283; Coverage: 100%)
Gene names: MSAP, MTAP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand 4CT 2 x 4CT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P63
Unit cell:
a: 121.539Å b: 121.539Å c: 87.661Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.2 0.256
Expression system: Escherichia coli BL21(DE3)

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