› 3p0g

X-ray diffraction
3.5Å resolution

Structure of a nanobody-stabilized active state of the beta2 adrenoceptor

Released:
DOI: 10.2210/pdb3p0g/pdb
PDB entry 3p0g coloured by chain, front view.
PDB entry 3p0g coloured by chain, side view.
PDB entry 3p0g coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a nanobody-stabilized active state of the β(2) adrenoceptor.
Rasmussen SG, Choi HJ, Fung JJ, Pardon E, Casarosa P, Chae PS, Devree BT, Rosenbaum DM, Thian FS, Kobilka TS, Schnapp A, Konetzki I, Sunahara RK, Gellman SH, Pautsch A, Steyaert J, Weis WI, Kobilka BK
Nature 469 175-80 (2011)
PMID: 21228869

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208289 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-2 adrenergic receptor; Endolysin Chain: A
Molecule details ›
Chain: A
Length: 501 amino acids
Theoretical weight: 56.72 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07550 (Residues: 1-230, 264-365; Coverage: 80%)
  • Canonical: P00720 (Residues: 2-162; Coverage: 98%)
Gene names: ADRB2, ADRB2R, B2AR, E
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins
Camelid Antibody Fragment Chain: B
Molecule details ›
Chain: B
Length: 126 amino acids
Theoretical weight: 13.78 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand P0G 1 x P0G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 236.686Å b: 45.66Å c: 71.375Å
α: 90° β: 102.34° γ: 90°
R-values:
R R work R free
0.243 0.235 0.308
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

254 review citations

Nanobodies: natural single-domain antibodies.
Muyldermans S. (2013)
253 more

167 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
166 more