› 3p0p

X-ray diffraction
2.49Å resolution

Human Tankyrase 2 - Catalytic PARP domain in complex with an inhibitor

Released:
DOI: 10.2210/pdb3p0p/pdb
PDB entry 3p0p coloured by chain, front view.
PDB entry 3p0p coloured by chain, side view.
PDB entry 3p0p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Family-wide chemical profiling and structural analysis of PARP and tankyrase inhibitors.
Wahlberg E, Karlberg T, Kouznetsova E, Markova N, Macchiarulo A, Thorsell AG, Pol E, Frostell Å, Ekblad T, Öncü D, Kull B, Robertson GM, Pellicciari R, Schüler H, Weigelt J
Nat Biotechnol 30 283-8 (2012)
PMID: 22343925

Function and Biology Details

Reaction catalysed: 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190549 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly [ADP-ribose] polymerase tankyrase-2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 240 amino acids
Theoretical weight: 27.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H2K2 (Residues: 946-1162; Coverage: 19%)
Gene names: PARP5B, TANK2, TNKL, TNKS2
Sequence domains: Poly(ADP-ribose) polymerase catalytic domain
Structure domains: Phosphoenolpyruvate Carboxykinase; domain 3

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand NNF 2 x NNF
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 90.647Å b: 97.892Å c: 118.211Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.189 0.261
Expression system: Escherichia coli

Quick links

Citations

92 review citations

New insights into the molecular and cellular functions of poly(ADP-ribose) and PARPs.
Gibson et al. (2012)
91 more

2 mentions without citation

Wnt/beta-catenin signaling and small molecule inhibitors.
Voronkov et al. (2013)
1 more