› 3p1d

X-ray diffraction
1.86Å resolution

Crystal structure of the bromodomain of human CREBBP in complex with N-Methyl-2-pyrrolidone (NMP)

Released:
DOI: 10.2210/pdb3p1d/pdb
PDB entry 3p1d coloured by chain, front view.
PDB entry 3p1d coloured by chain, side view.
PDB entry 3p1d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Histone recognition and large-scale structural analysis of the human bromodomain family.
Filippakopoulos P, Picaud S, Mangos M, Keates T, Lambert JP, Barsyte-Lovejoy D, Felletar I, Volkmer R, Müller S, Pawson T, Gingras AC, Arrowsmith CH, Knapp S
Cell 149 214-31 (2012)
PMID: 22464331

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CREB-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 119 amino acids
Theoretical weight: 14.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92793 (Residues: 1081-1197; Coverage: 5%)
Gene names: CBP, CREBBP
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

3 bound ligands:
Ligand SCN 1 x SCN
Ligand MB3 2 x MB3
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: R3
Unit cell:
a: 121.549Å b: 121.549Å c: 40.23Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.167 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

244 review citations

Modification of enhancer chromatin: what, how, and why?
Calo et al. (2013)
243 more

3 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
2 more