› 3p33

X-ray diffraction
2.3Å resolution

Insulin fibrillation is the Janus face of induced fit. A chiral clamp stabilizes the native state at the expense of activity

Released:
DOI: 10.2210/pdb3p33/pdb
PDB entry 3p33 coloured by chain, front view.
PDB entry 3p33 coloured by chain, side view.
PDB entry 3p33 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Hua QX, Wan ZL, Huang K, Hu SQ, Phillip NF, Jia WH, Whittingham J, Dodson GG, Katsoyannis PG, Weiss MA

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134224 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

3 bound ligands:
Ligand IPH 4 x IPH
Ligand ZN 4 x ZN
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: R3
Unit cell:
a: 77.9Å b: 77.9Å c: 78.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.299
Expression system: Not provided

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