› 3p49

X-ray diffraction
3.55Å resolution

Crystal Structure of a Glycine Riboswitch from Fusobacterium nucleatum

Released:
DOI: 10.2210/pdb3p49/pdb
PDB entry 3p49 coloured by chain, front view.
PDB entry 3p49 coloured by chain, side view.
PDB entry 3p49 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of cooperative ligand binding by the glycine riboswitch.
Butler EB, Xiong Y, Wang J, Strobel SA
Chem Biol 18 293-8 (2011)
PMID: 21439473

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140456 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U1 small nuclear ribonucleoprotein A Chain: B
Molecule details ›
Chain: B
Length: 98 amino acids
Theoretical weight: 11.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P09012 (Residues: 1-98; Coverage: 35%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
GLYCINE RIBOSWITCH Chain: A
Molecule details ›
Chain: A
Length: 169 nucleotides
Theoretical weight: 54.66 KDa
Source organism: Fusobacterium nucleatum subsp. polymorphum
Expression system: Not provided
Sequence domains: Glycine riboswitch

Ligands and Environments

2 bound ligands:
Ligand MG 13 x MG
Ligand GLY 2 x GLY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25, APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 54.58Å b: 104.617Å c: 156.509Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.284 0.282 0.31
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

14 review citations

A decade of riboswitches.
Serganov et al. (2013)
13 more

21 mentions without citation

Understanding nucleic acid-ion interactions.
Lipfert et al. (2014)
20 more