› 3p8d

X-ray diffraction
2Å resolution

Crystal structure of the second Tudor domain of human PHF20 (homodimer form)

Released:
DOI: 10.2210/pdb3p8d/pdb
PDB entry 3p8d coloured by chain, front view.
PDB entry 3p8d coloured by chain, side view.
PDB entry 3p8d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
PHF20 is an effector protein of p53 double lysine methylation that stabilizes and activates p53.
Cui G, Park S, Badeaux AI, Kim D, Lee J, Thompson JR, Yan F, Kaneko S, Yuan Z, Botuyan MV, Bedford MT, Cheng JQ, Mer G
Nat Struct Mol Biol 19 916-24 (2012)
PMID: 22864287

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189761 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PHD finger protein 20 Chains: A, B
Molecule details ›
Chains: A, B
Length: 67 amino acids
Theoretical weight: 7.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BVI0 (Residues: 84-147; Coverage: 6%)
Gene names: C20orf104, GLEA2, HCA58, NZF, PHF20, TZP
Sequence domains: DNA repair protein Crb2 Tudor domain
Structure domains: SH3 type barrels.

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P43
Unit cell:
a: 48.548Å b: 48.548Å c: 96.149Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.262
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Non-histone protein methylation as a regulator of cellular signalling and function.
Biggar et al. (2015)
19 more

5 mentions without citation

G9a-mediated methylation of ERĪ± links the PHF20/MOF histone acetyltransferase complex to hormonal gene expression.
Zhang et al. (2016)
4 more