› 3pcv

X-ray diffraction
1.9Å resolution

Crystal structure analysis of human leukotriene C4 synthase at 1.9 angstrom resolution

Released:
DOI: 10.2210/pdb3pcv/pdb
PDB entry 3pcv coloured by chain, front view.
PDB entry 3pcv coloured by chain, side view.
PDB entry 3pcv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The catalytic architecture of leukotriene C4 synthase with two arginine residues.
Saino H, Ukita Y, Ago H, Irikura D, Nisawa A, Ueno G, Yamamoto M, Kanaoka Y, Lam BK, Austen KF, Miyano M
J Biol Chem 286 16392-401 (2011)
PMID: 21454538

Function and Biology Details

Reaction catalysed: 
Leukotriene C(4) = leukotriene A(4) + glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172606 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leukotriene C4 synthase Chain: A
Molecule details ›
Chain: A
Length: 156 amino acids
Theoretical weight: 17.41 KDa
Source organism: Homo sapiens
Expression system: Schizosaccharomyces pombe
UniProt:
  • Canonical: Q16873 (Residues: 1-150; Coverage: 100%)
Gene name: LTC4S
Sequence domains: MAPEG family
Structure domains: Membrane associated eicosanoid/glutathione metabolism-like domain

Ligands and Environments


Cofactor: Ligand GSH 1 x GSH
4 bound ligands:
Ligand LMT 11 x LMT
Ligand EDO 4 x EDO
Ligand UNL 4 x UNL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: F23
Unit cell:
a: 168.03Å b: 168.03Å c: 168.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.198
Expression system: Schizosaccharomyces pombe

Quick links

Citations

7 review citations

Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes.
Deponte M. (2013)
6 more

4 mentions without citation

Crystal structures of leukotriene C4 synthase in complex with product analogs: implications for the enzyme mechanism.
Niegowski et al. (2014)
3 more