› 3pfw

X-ray diffraction
2.15Å resolution

Crystal structure of human sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) complex with NAD, a binary form

Released:
DOI: 10.2210/pdb3pfw/pdb
PDB entry 3pfw coloured by chain, front view.
PDB entry 3pfw coloured by chain, side view.
PDB entry 3pfw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and kinetic characterization of human sperm-specific glyceraldehyde-3-phosphate dehydrogenase, GAPDS.
Chaikuad A, Shafqat N, Al-Mokhtar R, Cameron G, Clarke AR, Brady RL, Oppermann U, Frayne J, Yue WW
Biochem J 435 401-9 (2011)
PMID: 21269272

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific Chains: O, P
Molecule details ›
Chains: O, P
Length: 346 amino acids
Theoretical weight: 37.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14556 (Residues: 69-407; Coverage: 83%)
Gene names: GAPD2, GAPDH2, GAPDHS, GAPDS, HSD-35, HSD35
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 143.61Å b: 71.86Å c: 81.1Å
α: 90° β: 123.32° γ: 90°
R-values:
R R work R free
0.17 0.168 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Deregulation of glycolysis in cancer: glyceraldehyde-3-phosphate dehydrogenase as a therapeutic target.
Krasnov et al. (2013)
2 more

3 mentions without citation

Development and Implementation of a High Throughput Screen for the Human Sperm-Specific Isoform of Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDHS).
Sexton et al. (2011)
2 more