› 3pkn

X-ray diffraction
1.8Å resolution

Crystal structure of MLLE domain of poly(A) binding protein in complex with PAM2 motif of La-related protein 4 (LARP4)

Released:
DOI: 10.2210/pdb3pkn/pdb
PDB entry 3pkn coloured by chain, front view.
PDB entry 3pkn coloured by chain, side view.
PDB entry 3pkn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
La-related protein 4 binds poly(A), interacts with the poly(A)-binding protein MLLE domain via a variant PAM2w motif, and can promote mRNA stability.
Yang R, Gaidamakov SA, Xie J, Lee J, Martino L, Kozlov G, Crawford AK, Russo AN, Conte MR, Gehring K, Maraia RJ
Mol Cell Biol 31 542-56 (2011)
PMID: 21098120

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-145993 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polyadenylate-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 9.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11940 (Residues: 544-626; Coverage: 13%)
Gene names: PAB1, PABP, PABP1, PABPC1, PABPC2
Sequence domains: Poly-adenylate binding protein, unique domain
Structure domains: c-terminal domain of poly(a) binding protein
La-related protein 4 Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.54 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q71RC2 (Residues: 13-26; Coverage: 2%)
Gene names: LARP4, PP13296

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand IOD 1 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 48.91Å b: 31.384Å c: 52.647Å
α: 90° β: 104.95° γ: 90°
R-values:
R R work R free
0.227 0.225 0.269
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Handshakes and Fights: The Regulatory Interplay of RNA-Binding Proteins.
Dassi E. (2017)
9 more

5 mentions without citation

LARP1 and LARP4: up close with PABP for mRNA 3' poly(A) protection and stabilization.
Mattijssen et al. (2021)
4 more