› 3psc

X-ray diffraction
2.67Å resolution

Bovine GRK2 in complex with Gbetagamma subunits

Released:
DOI: 10.2210/pdb3psc/pdb
PDB entry 3psc coloured by chain, front view.
PDB entry 3psc coloured by chain, side view.
PDB entry 3psc coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Molecular mechanism of selectivity among G protein-coupled receptor kinase 2 inhibitors.
Thal DM, Yeow RY, Schoenau C, Huber J, Tesmer JJ
Mol Pharmacol 80 294-303 (2011)
PMID: 21596927

Function and Biology Details

Reaction catalysed: 
ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-149112 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-adrenergic receptor kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 695 amino acids
Theoretical weight: 80.58 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21146 (Residues: 1-689; Coverage: 100%)
Gene names: ADRBK1, GRK2
Sequence domains:
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 74 amino acids
Theoretical weight: 8.41 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-68; Coverage: 96%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CMT 1 x CMT

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 187.928Å b: 73.152Å c: 122.78Å
α: 90° β: 115.42° γ: 90°
R-values:
R R work R free
0.225 0.223 0.257
Expression system: Spodoptera frugiperda

Quick links

Citations

22 review citations

G protein-coupled receptor kinases: more than just kinases and not only for GPCRs.
Gurevich et al. (2012)
21 more

3 mentions without citation

Structural insights into G protein-coupled receptor kinase function.
Homan et al. (2014)
2 more