› 3ptk

X-ray diffraction
2.49Å resolution

The crystal structure of rice (Oryza sativa L.) Os4BGlu12

Released:
DOI: 10.2210/pdb3ptk/pdb
PDB entry 3ptk coloured by chain, front view.
PDB entry 3ptk coloured by chain, side view.
PDB entry 3ptk coloured by chain, top view.
Source organism: Oryza sativa
Primary publication:
The crystal structure of rice (Oryza sativa L.) Os4BGlu12, an oligosaccharide and tuberonic acid glucoside-hydrolyzing β-glucosidase with significant thioglucohydrolase activity.
Sansenya S, Opassiri R, Kuaprasert B, Chen CJ, Cairns JR
Arch Biochem Biophys 510 62-72 (2011)
PMID: 21521631

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase 12 Chains: A, B
Molecule details ›
Chains: A, B
Length: 505 amino acids
Theoretical weight: 57.23 KDa
Source organism: Oryza sativa
Expression system: Escherichia coli
UniProt:
  • Canonical: B8AVF0 (Residues: 25-510; Coverage: 100%)
Gene names: BGLU12, OSIGBa0135C13.7, OsI_16288
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand TRS 2 x TRS
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P43212
Unit cell:
a: 112.656Å b: 112.656Å c: 182.773Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.248
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

Expression of the β-glucosidase gene Pgβglu-1 underpins natural resistance of white spruce against spruce budworm.
Mageroy et al. (2015)
10 more

4 mentions without citation

Functional Characterization of CsBGlu12, a β-Glucosidase from Crocus sativus, Provides Insights into Its Role in Abiotic Stress through Accumulation of Antioxidant Flavonols.
Baba et al. (2017)
3 more