› 3q18

X-ray diffraction
1.7Å resolution

Human Glutathione Transferase O2

Released:
DOI: 10.2210/pdb3q18/pdb
PDB entry 3q18 coloured by chain, front view.
PDB entry 3q18 coloured by chain, side view.
PDB entry 3q18 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the dehydroascorbate reductase activity of human omega-class glutathione transferases.
Zhou H, Brock J, Liu D, Board PG, Oakley AJ
J Mol Biol 420 190-203 (2012)
PMID: 22522127

Function and Biology Details

Reactions catalysed: 
Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H(2)O
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase omega-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H4Y5 (Residues: 1-239; Coverage: 98%)
Gene name: GSTO2
Sequence domains: Glutathione S-transferase, N-terminal domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PEG 1 x PEG
Ligand GOL 1 x GOL
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 46.659Å b: 85.718Å c: 60.359Å
α: 90° β: 95.26° γ: 90°
R-values:
R R work R free
0.159 0.157 0.186
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Human genetic variation influences vitamin C homeostasis by altering vitamin C transport and antioxidant enzyme function.
Michels et al. (2013)
4 more

3 mentions without citation

A role for glutathione transferase Omega 1 (GSTO1-1) in the glutathionylation cycle.
Menon et al. (2013)
2 more