› 3q29

X-ray diffraction
2.3Å resolution

Cyrstal structure of human alpha-synuclein (1-19) fused to maltose binding protein (MBP)

Released:
DOI: 10.2210/pdb3q29/pdb
PDB entry 3q29 coloured by chain, front view.
PDB entry 3q29 coloured by chain, side view.
PDB entry 3q29 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of segments of α-synuclein fused to maltose-binding protein suggest intermediate states during amyloid formation.
Zhao M, Cascio D, Sawaya MR, Eisenberg D
Protein Sci 20 996-1004 (2011)
PMID: 21462277

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-synuclein; Maltose/maltodextrin-binding periplasmic protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 390 amino acids
Theoretical weight: 42.7 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: P37840 (Residues: 1-19; Coverage: 14%)
Gene names: JW3994, NACP, PARK1, SNCA, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand GOL 4 x GOL
Ligand SO4 11 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 68.39Å b: 117.47Å c: 146.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.232
Expression system: Escherichia coli

Quick links

Citations

17 citation in other articles

A soluble α-synuclein construct forms a dynamic tetramer.
Wang et al. (2011)
16 more

81 mentions without citation

Xenobiotic, bile acid, and cholesterol transporters: function and regulation.
Klaassen et al. (2010)
80 more