› 3q2t

X-ray diffraction
3.06Å resolution

Crystal Structure of CFIm68 RRM/CFIm25/RNA complex

Released:
DOI: 10.2210/pdb3q2t/pdb
PDB entry 3q2t coloured by chain, front view.
PDB entry 3q2t coloured by chain, side view.
PDB entry 3q2t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a human cleavage factor CFI(m)25/CFI(m)68/RNA complex provides an insight into poly(A) site recognition and RNA looping.
Yang Q, Coseno M, Gilmartin GM, Doublié S
Structure 19 368-77 (2011)
PMID: 21295486

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129137 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Cleavage and polyadenylation specificity factor subunit 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 24.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43809 (Residues: 21-227; Coverage: 91%)
Gene names: CFIM25, CPSF25, CPSF5, NUDT21
Sequence domains: Nucleotide hydrolase
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase
Cleavage and polyadenylation specificity factor subunit 6 Chains: C, D
Molecule details ›
Chains: C, D
Length: 229 amino acids
Theoretical weight: 24.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16630 (Residues: 13-235; Coverage: 41%)
Gene names: CFIM68, CPSF6
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA Chains: E, F
Molecule details ›
Chains: E, F
Length: 5 nucleotides
Theoretical weight: 1.55 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P213
Unit cell:
a: 138.406Å b: 138.406Å c: 138.406Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.211 0.286
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Alternative cleavage and polyadenylation: extent, regulation and function.
Elkon et al. (2013)
22 more

6 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
5 more