› 3q52

X-ray diffraction
1.8Å resolution

Structure of phosphorylated PAK1 kinase domain

Released:
DOI: 10.2210/pdb3q52/pdb
PDB entry 3q52 coloured by chain, front view.
PDB entry 3q52 coloured by chain, side view.
PDB entry 3q52 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the autoactivation mechanism of p21-activated protein kinase.
Wang J, Wu JW, Wang ZX
Structure 19 1752-61 (2011)
PMID: 22153498

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PAK 1 Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13153 (Residues: 248-545; Coverage: 55%)
Gene name: PAK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 52.621Å b: 103.054Å c: 122.596Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.188 0.214
Expression system: Escherichia coli

Quick links

Citations

13 review citations

How Do Protein Kinases Take a Selfie (Autophosphorylate)?
Beenstock et al. (2016)
12 more

4 mentions without citation

Thymoquinone-induced conformational changes of PAK1 interrupt prosurvival MEK-ERK signaling in colorectal cancer.
El-Baba et al. (2014)
3 more