› 3qag

X-ray diffraction
2Å resolution

Human Glutathione Transferase O2 with glutathione -new crystal form

Released:
DOI: 10.2210/pdb3qag/pdb
PDB entry 3qag coloured by chain, front view.
PDB entry 3qag coloured by chain, side view.
PDB entry 3qag coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the dehydroascorbate reductase activity of human omega-class glutathione transferases.
Zhou H, Brock J, Liu D, Board PG, Oakley AJ
J Mol Biol 420 190-203 (2012)
PMID: 22522127

Function and Biology Details

Reactions catalysed: 
Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H(2)O
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase omega-2 Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H4Y5 (Residues: 1-239; Coverage: 98%)
Gene name: GSTO2
Sequence domains: Glutathione S-transferase, N-terminal domain
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 1 x GSH
2 bound ligands:
Ligand PEG 2 x PEG
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P6522
Unit cell:
a: 53.138Å b: 53.138Å c: 352.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.224 0.265
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Human genetic variation influences vitamin C homeostasis by altering vitamin C transport and antioxidant enzyme function.
Michels et al. (2013)
4 more

1 mention without citation

Clonorchis sinensis omega-class glutathione transferases play major roles in the protection of the reproductive system during maturation and the response to oxidative stress.
Kim et al. (2016)