› 3qfj

X-ray diffraction
2.29Å resolution

The complex between TCR A6 and human Class I MHC HLA-A2 with the modified TAX (Y5F) peptide

Released:
DOI: 10.2210/pdb3qfj/pdb
PDB entry 3qfj coloured by chain, front view.
PDB entry 3qfj coloured by chain, side view.
PDB entry 3qfj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Disparate degrees of hypervariable loop flexibility control T-cell receptor cross-reactivity, specificity, and binding mechanism.
Scott DR, Borbulevych OY, Piepenbrink KH, Corcelli SA, Baker BM
J Mol Biol 414 385-400 (2011)
PMID: 22019736

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209036 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
TAX(Y5F) peptide Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.05 KDa
A6 alpha chain Chain: D
Molecule details ›
Chain: D
Length: 200 amino acids
Theoretical weight: 22.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
A6 beta chain Chain: E
Molecule details ›
Chain: E
Length: 245 amino acids
Theoretical weight: 27.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand GOL 11 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 224.008Å b: 48.098Å c: 92.959Å
α: 90° β: 91.02° γ: 90°
R-values:
R R work R free
0.221 0.218 0.271
Expression system: Escherichia coli

Quick links

Citations

13 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
12 more

5 mentions without citation

Immunoglobulin and T Cell Receptor Genes: IMGT(®) and the Birth and Rise of Immunoinformatics.
Lefranc MP. (2014)
4 more