› 3qj4

X-ray diffraction
2.5Å resolution

Crystal structure of Human Renalase (isoform 1)

Released:
DOI: 10.2210/pdb3qj4/pdb
PDB entry 3qj4 coloured by chain, front view.
PDB entry 3qj4 coloured by chain, side view.
PDB entry 3qj4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
FAD-binding site and NADP reactivity in human renalase: a new enzyme involved in blood pressure regulation.
Milani M, Ciriello F, Baroni S, Pandini V, Canevari G, Bolognesi M, Aliverti A
J Mol Biol 411 463-73 (2011)
PMID: 21699903

Function and Biology Details

Reaction catalysed: 
1,2-dihydro-beta-NAD(P) + H(+) + O(2) = beta-NAD(P)(+) + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178455 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Renalase Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 37.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5VYX0 (Residues: 1-342; Coverage: 100%)
Gene names: C10orf59, RNLS
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 53.837Å b: 86.593Å c: 93.18Å
α: 90° β: 95.3° γ: 90°
R-values:
R R work R free
0.219 0.217 0.26
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Renalase in hypertension and kidney disease.
Desir et al. (2014)
9 more

4 mentions without citation

Genome-scale in vivo CRISPR screen identifies RNLS as a target for beta cell protection in type 1 diabetes.
Cai et al. (2020)
3 more