› 3qsv

X-ray diffraction
2.71Å resolution

Structural basis for DNA recognition by constitutive Smad4 MH1 dimers

Released:
DOI: 10.2210/pdb3qsv/pdb
PDB entry 3qsv coloured by chain, front view.
PDB entry 3qsv coloured by chain, side view.
PDB entry 3qsv coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Baburajendran N, Jauch R, Zhen CTY, Kolatkar PR

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116786 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Mothers against decapentaplegic homolog 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 132 amino acids
Theoretical weight: 15.02 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P97471 (Residues: 9-140; Coverage: 24%)
Gene names: Dpc4, Madh4, Smad4
Sequence domains: MH1 domain
Structure domains: SMAD MH1 domain
DNA (5'-D(P*TP*GP*CP*AP*GP*TP*CP*TP*AP*GP*AP*CP*TP*GP*CP*A)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 178.357Å b: 35.354Å c: 139.544Å
α: 90° β: 93.83° γ: 90°
R-values:
R R work R free
0.215 0.212 0.272
Expression systems:
  • Escherichia coli
  • Not provided

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