› 3qum

X-ray diffraction
3.2Å resolution

Crystal structure of human prostate specific antigen (PSA) in Fab sandwich with a high affinity and a PCa selective antibody

Released:
DOI: 10.2210/pdb3qum/pdb
PDB entry 3qum coloured by chain, front view.
PDB entry 3qum coloured by chain, side view.
PDB entry 3qum coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of human prostate-specific antigen in a sandwich antibody complex.
Stura EA, Muller BH, Bossus M, Michel S, Jolivet-Reynaud C, Ducancel F
J Mol Biol 414 530-44 (2011)
PMID: 22037582

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: -Tyr-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209307 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (8 distinct):
Prostate-specific antigen Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 237 amino acids
Theoretical weight: 26.12 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07288 (Residues: 25-261; Coverage: 97%)
Gene names: APS, KLK3
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Fab 5D3D11 Light Chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 219 amino acids
Theoretical weight: 24.27 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab 5D3D11 Heavy Chain Chains: H, K
Molecule details ›
Chains: H, K
Length: 219 amino acids
Theoretical weight: 23.55 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab 5D5A5 Light Chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 218 amino acids
Theoretical weight: 24.19 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab 5D5A5 Heavy Chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 219 amino acids
Theoretical weight: 23.57 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN, GAL, SIA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN, GAL, SIA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, A2G
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 55.308Å b: 226.883Å c: 118.694Å
α: 90° β: 96.51° γ: 90°
R-values:
R R work R free
0.203 0.198 0.294

Quick links

Citations

10 review citations

Aberrant PSA glycosylation--a sweet predictor of prostate cancer.
Gilgunn et al. (2013)
9 more

15 mentions without citation

Multifaceted Role of Sialylation in Prion Diseases.
Baskakov et al. (2016)
14 more