› 3rau

X-ray diffraction
1.95Å resolution

Crystal structure of the HD-PTP Bro1 domain

Released:
DOI: 10.2210/pdb3rau/pdb
PDB entry 3rau coloured by chain, front view.
PDB entry 3rau coloured by chain, side view.
PDB entry 3rau coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Phe105 loop of Alix Bro1 domain plays a key role in HIV-1 release.
Sette P, Mu R, Dussupt V, Jiang J, Snyder G, Smith P, Xiao TS, Bouamr F
Structure 19 1485-95 (2011)
PMID: 21889351

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190592 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 23 Chains: A, B
Molecule details ›
Chains: A, B
Length: 363 amino acids
Theoretical weight: 40.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H3S7 (Residues: 2-361; Coverage: 22%)
Gene names: KIAA1471, PTPN23
Sequence domains: BRO1-like domain
Structure domains: alix/aip1 like domains

Ligands and Environments

3 bound ligands:
Ligand ACT 8 x ACT
Ligand EDO 2 x EDO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 37.523Å b: 61.333Å c: 80.582Å
α: 85.87° β: 81.27° γ: 87.99°
R-values:
R R work R free
0.188 0.172 0.215
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Membrane fission reactions of the mammalian ESCRT pathway.
McCullough et al. (2013)
2 more

6 mentions without citation

The Phe105 loop of Alix Bro1 domain plays a key role in HIV-1 release.
Sette et al. (2011)
5 more