› 3rew

X-ray diffraction
1.9Å resolution

Crystal structure of an lmp2a-derived peptide bound to human class i mhc hla-a2

Released:
DOI: 10.2210/pdb3rew/pdb
PDB entry 3rew coloured by chain, front view.
PDB entry 3rew coloured by chain, side view.
PDB entry 3rew coloured by chain, top view.
Source organisms:
Primary publication:
Structural and energetic evidence for highly peptide-specific tumor antigen targeting via allo-MHC restriction.
Simpson AA, Mohammed F, Salim M, Tranter A, Rickinson AB, Stauss HJ, Moss PA, Steven NM, Willcox BE
Proc Natl Acad Sci U S A 108 21176-81 (2011)
PMID: 22160697

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137664 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Latent membrane protein 2 Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 906 Da
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
  • Canonical: P13285 (Residues: 426-434; Coverage: 2%)
Gene name: LMP2

Ligands and Environments

3 bound ligands:
Ligand IOD 8 x IOD
Ligand EDO 2 x EDO
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 49.75Å b: 141.44Å c: 141.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.239
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Challenges in T cell receptor gene therapy.
Uttenthal et al. (2012)
2 more

5 mentions without citation

Broad TCR repertoire and diverse structural solutions for recognition of an immunodominant CD8+ T cell epitope.
Song et al. (2017)
4 more