› 3rip

X-ray diffraction
2.3Å resolution

Crystal Structure of human gamma-tubulin complex protein 4 (GCP4)

Released:
DOI: 10.2210/pdb3rip/pdb
PDB entry 3rip coloured by chain, front view.
PDB entry 3rip coloured by chain, side view.
PDB entry 3rip coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of γ-tubulin complex protein GCP4 provides insight into microtubule nucleation.
Guillet V, Knibiehler M, Gregory-Pauron L, Remy MH, Chemin C, Raynaud-Messina B, Bon C, Kollman JM, Agard DA, Merdes A, Mourey L
Nat Struct Mol Biol 18 915-9 (2011)
PMID: 21725292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-193711 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-tubulin complex component 4 Chain: A
Molecule details ›
Chain: A
Length: 677 amino acids
Theoretical weight: 77.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UGJ1 (Residues: 1-667; Coverage: 100%)
Gene names: 76P, GCP4, TUBGCP4
Sequence domains:
Structure domains: Gamma-tubulin complex, C-terminal domain

Ligands and Environments

2 bound ligands:
Ligand MRD 1 x MRD
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P6322
Unit cell:
a: 214.95Å b: 214.95Å c: 128.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.227 0.26
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Microtubule nucleation by γ-tubulin complexes.
Kollman et al. (2011)
9 more

8 mentions without citation

Asymmetric Molecular Architecture of the Human γ-Tubulin Ring Complex.
Wieczorek et al. (2020)
7 more