› 3ris

X-ray diffraction
2.4Å resolution

Crystal structure of the catalytic domain of UCHL5, a proteasome-associated human deubiquitinating enzyme, reveals an unproductive form of the enzyme

Released:
DOI: 10.2210/pdb3ris/pdb
PDB entry 3ris coloured by chain, front view.
PDB entry 3ris coloured by chain, side view.
PDB entry 3ris coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the catalytic domain of UCHL5, a proteasome-associated human deubiquitinating enzyme, reveals an unproductive form of the enzyme.
Maiti TK, Permaul M, Boudreaux DA, Mahanic C, Mauney S, Das C
FEBS J 278 4917-26 (2011)
PMID: 21995438

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-195274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase isozyme L5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 245 amino acids
Theoretical weight: 27.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5K5 (Residues: 1-240; Coverage: 73%)
Gene names: AD-019, CGI-70, UCH37, UCHL5
Sequence domains: Ubiquitin carboxyl-terminal hydrolase, family 1
Structure domains: Peptidase C12, ubiquitin carboxyl-terminal hydrolase

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 87.393Å b: 41.67Å c: 138.729Å
α: 90° β: 89.93° γ: 90°
R-values:
R R work R free
0.204 0.201 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Deubiquitinases (DUBs) and DUB inhibitors: a patent review.
Farshi et al. (2015)
4 more

10 mentions without citation

Clinically used antirheumatic agent auranofin is a proteasomal deubiquitinase inhibitor and inhibits tumor growth.
Liu et al. (2014)
9 more